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dc.contributor.authorCalzada, Javier
dc.contributor.authorAlcón, A.
dc.contributor.authorSantos, Victoria E.
dc.contributor.authorGarcía-Ochoa, Félix
dc.date.accessioned2014-09-01T08:21:58Z
dc.date.available2014-09-01T08:21:58Z
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/10641/1002
dc.description.abstractSulfur contentinfossilfuelsisknowntobethemostimportantanthropogeniccauseofsulfuroxide emissions totheatmosphere.Inordertoavoidhealth,environmentalandtechnicalproblemscaused by thiscompound,legislationimposesrestrictivelimitationstofuelsulfurcontent.Biodesulfurization (BDS) canbecomeacomplementarytechnologytohydrodesulfurization(HDS)tofacethissituation. Pseudomonas putida CECT 5279isageneticallymodifiedmicroorganismwhichcanactasadesulfurizing biocatalyst. Thismicroorganismhastheabilityofperformingthemetabolic4Spathwayfrom Rhodococcus erythropolis IGTS8, inordertodesulfurizeDBT,asamodelcompound.Maximum in vivo activities of monooxygenase enzymes(DszAandDszC)areshownwhenlateexponentialgrowthphaseisreached (23 h),whiledesulfinaseenzymeDszBpresentsamaximumactivityduringtheearlyexponentialgrowth phase (5h),aspreviouslyreported [1]. Also,ithasbeenprovedthatthecombinedutilizationofthesetwo cell agesyieldsexcellentresultswhenusedasbiocatalystfordesulfurization [2]. Theaimofthisworkis to optimizetheratioandtotalbiomassconcentrationofboth5hand23hgrowthtimecellsinacomplex biocatalyst fordesulfurizationbyperformingrestingcellsbiodesulfurizationassaysusingdibenzothio- phene (DBT)assulfurmodelcompound.Thebestcombinationofcellswasdeterminedaimingforthe highest desulfurizationintheshortesttimeofoperationwhileinvestingtheminimumconcentrationof biomass. Aparticularcellmixture,containing66.7%of23hgrowthtimecells,wasfoundtoworkasthe most effectivedesulfurizationbiocatalyst.spa
dc.language.isoengspa
dc.publisherProcess Biochemistry, 46spa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleMixtures of Pseudomonas putida CECT 5279 cells of different ages: optimization as biodesulfurization catalyst.spa
dc.typearticlespa
dc.description.versionpre-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent495 KBspa


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España