Sustained Human Hair Follicle Growth Ex Vivo in a Glycosaminoglycan Hydrogel Matrix.
Loading...
Identifiers
Publication date
2019
Start date of the public exhibition period
End date of the public exhibition period
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Molecular Sciences
Metrics
Abstract
Glycosaminoglycans (GAGs) and associated proteoglycans have important functions in
homeostatic maintenance and regenerative processes (e.g., wound repair) of the skin. However,
little is known about the role of these molecules in the regulation of the hair follicle cycle. Here we
report that growing human hair follicles ex vivo in a defined GAG hydrogel mimicking the dermal
matrix strongly promotes sustained cell survival and maintenance of a highly proliferative
phenotype in the hair bulb and suprabulbar regions. This significant effect is associated with the
activation of WNT/β-catenin signaling targets (CCDN1, AXIN2) and with the expression of stem
cell markers (CK15, CD34) and growth factors implicated in the telogen/anagen transition (TGFβ2,
FGF10). As a whole, these results point to the dermal GAG matrix as an important component in
the regulation of the human hair follicle growth cycle, and to GAG-based hydrogels as potentially
relevant modulators of this process both in vitro and in vivo.
Doctoral program
Description
Keywords
Glycosaminoglycans, Hyaluronic acid, Stem cells, Human hair follicl