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dc.contributor.authorHsieh, Vivian
dc.contributor.authorOkada, Satoshi
dc.contributor.authorWe, He
dc.contributor.authorGarcía Álvarez, Isabel
dc.contributor.authorBarandov, Ali
dc.contributor.authorRecuenco Alvarado, Santiago
dc.contributor.authorOhlendorf, Robert
dc.contributor.authorFan, Jingxuan
dc.contributor.authorOrtega, Athena
dc.contributor.authorJasanoff, Alan
dc.date.accessioned2019-11-04T08:54:00Z
dc.date.available2019-11-04T08:54:00Z
dc.date.issued2019
dc.identifier.issn0002-7863spa
dc.identifier.urihttp://hdl.handle.net10641/1709
dc.description.abstractNeurotransmitter-sensitive contrast agents for magnetic resonance imaging (MRI) have recently been used for mapping signaling dynamics in live animal brains, but paramagnetic sensors for T1-weighted MRI are usually effective only at micromolar concentrations that themselves perturb neurochemistry. Here we present an alternative molecular architecture for detecting neurotransmitters, using superparamagnetic iron oxide nanoparticles conjugated to tethered neurotransmitter analogs and engineered neurotransmitter binding proteins. Interactions between the nanoparticle conjugates result in clustering that is reversibly disrupted in the presence of neurotransmitter analytes, thus altering T2-weighted MRI signals. We demonstrate this principle using tethered dopamine and serotonin analogs, together with proteins selected for their ability to competitively bind either the analogs or the neurotransmitters themselves. Corresponding sensors for dopamine and serotonin exhibit target-selective relaxivity changes of up to 20%, while also operating below endogenous neurotransmitter concentrations. Semisynthetic magnetic particle sensors thus represent a promising path for minimally perturbative studies of neurochemical analytes.spa
dc.language.isoengspa
dc.publisherJournal of the American Chemical Societyspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleNeurotransmitter-Responsive Nanosensors for T2‑Weighted Magnetic Resonance Imaging.spa
dc.typearticlespa
dc.description.versionpost-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent2482 KBspa
dc.identifier.doi10.1021/jacs.9b08744spa
dc.relation.publisherversionhttps://pubs.acs.org/doi/full/10.1021/jacs.9b08744spa


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España