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dc.contributor.authorGil Ranedo, Jon
dc.contributor.authorHernando, Eva
dc.contributor.authorValle Benítez, Noelia
dc.contributor.authorRiolobos, Laura
dc.contributor.authorMaroto, Beatriz
dc.contributor.authorAlmendral, José M.
dc.date.accessioned2019-11-05T11:18:26Z
dc.date.available2019-11-05T11:18:26Z
dc.date.issued2018
dc.identifier.issn0042-6822spa
dc.identifier.urihttp://hdl.handle.net10641/1717
dc.description.abstractThe T1 parvovirus Minute Virus of Mice (MVM) was used to study the roles that phosphorylation and N-terminal domains (Nt) configuration of capsid subunits may play in icosahedral nuclear viruses assembly. In synchronous MVM infection, capsid subunits newly assembled as two types of cytoplasmic trimeric intermediates (3VP2, and 1VP1:2VP2) harbored a VP1 phosphorylation level fivefold higher than that of VP2, and hidden Nt. Upon nuclear translocation at S phase, VP1-Nt became exposed in the heterotrimer and subsequent subviral assembly intermediates. Empty capsid subunits showed a phosphorylation level restored to VP1:VP2 stoichiometry, and the Nt concealed in their interior. However ssDNA-filled virus maturing at S/G2 lacked VP1 phosphorylation and one major VP2 phosphopeptide, and exposed VP2-Nt. Endosomal VP2-Nt cleavage resulted in VP3 subunits devoid of any phospholabel, implying that incoming viral particles specifically harbor a low phosphorylation status. Phosphorylation provides a mechanistic coupling of parvovirus nuclear assembly to the cell cycle.spa
dc.language.isoengspa
dc.publisherVirologyspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectAssemblyspa
dc.subjectCapsid phosphorylationspa
dc.subjectMaturationspa
dc.subjectParvovirusspa
dc.subjectViral traffickingspa
dc.titleDifferential Phosphorylation and N-terminal Configuration of Capsid Subunits in Parvovirus Assembly and Viral Trafficking.spa
dc.typearticlespa
dc.description.versionpre-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent1730 KBspa
dc.identifier.doi10.1016/j.virol.2018.02.018spa
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0042682218300618?via%3Dihubspa


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España