dc.description.abstract | Wine production is one of the oldest and most ingrained biotechnological
process in human history worth more than 4 billion euros in 2016. In the last
years, this ancient industry started a new era to adapt production to the interests
of consumers, who demand high quality natural and organic wines. The
employment of clear-cut methods of cultivation, harvesting and fermentation, as
stated within the framework of the “EU No 203/2012 of 8 March 2012” and the
use of indigenous yeasts from their cultivars and wineries, guarantee the
production of wines with unique flavor and aroma qualified by the
region's “terroir”. The focus of this work has been the study of the indigenous
yeasts isolated from the wineries Aster and Diaz Bayo from Ribera del Duero
(P.D.O). One thousand two hundred indigenous yeasts from the cellar and
grapes of the wineries were isolated and characterized using molecular
standardized methods (ITS-RFLP and sequencing of D1/D2 regions). Then, 43
wild yeasts were characterized using AFLP (Amplified Fragment Length
Polymorphism) molecular markers obtained with seven primer pairs fluorescent
labelled (FAM or HEX). Using the species and strain specific AFLP alleles
amplified with two primer sets (S and J) a database was created for the
identification of wine yeasts in must samples, without the use of microbial
cultures. In addition, fermentation experiments using Tempranillo´s commercial
wort were carried out with selected wild strains in order to observe their
influence in the production of organic compounds determining the wine
aroma. Three yeast strains, Lt1, Mp3 and Hu1, standed out since they produced
ethyl hexanoate, ethyl octanoate and 2-phenylethanol, which contribute to the
fruity (banana, peach notes) and floral (rose notes) aromas in Tempranillo wine. | spa |