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dc.contributor.authorBlesa Esteban, Alba
dc.contributor.authorSánchez, Mercedes
dc.contributor.authorSacristán-Horcajada, Eva
dc.contributor.authorGonzález-de la Fuente, Sandra
dc.contributor.authorPeiró, Ramón
dc.contributor.authorBerenguer, José
dc.date.accessioned2020-09-17T10:19:58Z
dc.date.available2020-09-17T10:19:58Z
dc.date.issued2019
dc.identifier.issn2076-2607spa
dc.identifier.urihttp://hdl.handle.net/10641/1970
dc.description.abstract: A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these habitats. In this work, active and inactive insertion sequences (ISs) spanning the sequenced members of the genus Thermus were characterized, with special emphasis on three T. thermophilus strains: HB27, HB8, and NAR1. A large number of full ISs and fragments derived from different IS families were found, concentrating within megaplasmids present in most isolates. Potentially active ISs were identified through analysis of transposase integrity, and domestication-related transposition events of ISTth7 were identified in laboratory-adapted HB27 derivatives. Many partial copies of ISs appeared throughout the genome, which may serve as specific targets for homologous recombination contributing to genome rearrangement. Moreover, recruitment of IS1000 32 bp segments as spacers for CRISPR sequence was identified, pointing to the adaptability of these elements in the biology of these thermophiles. Further knowledge about the activity and functional diversity of ISs in this genus may contribute to the generation of engineered transposons as new genetic tools, and enrich our understanding of the outstanding plasticity shown by these thermophiles.spa
dc.language.isoengspa
dc.publisherMDPIspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectInsertion sequencespa
dc.subjectTransposonsspa
dc.subjectTransposasesspa
dc.subjectThermusspa
dc.subjectThermophilesspa
dc.subjectMobilomespa
dc.titleInto the Thermus Mobilome: Presence, Diversity and Recent Activities of Insertion Sequences Across Thermus spp.spa
dc.typearticlespa
dc.description.versionpost-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent2.276 KBspa
dc.identifier.doi10.3390/microorganisms7010025spa
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/7/1/25spa


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