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dc.contributor.authorNieto-MárquezCalvo, Jorge
dc.contributor.authorElices, Manuel
dc.contributor.authorV.Guinea, Gustavo
dc.contributor.authorPérez-Rigueiro, José
dc.contributor.authorArroyo-Hernández, María
dc.date.accessioned2021-01-26T09:13:20Z
dc.date.available2021-01-26T09:13:20Z
dc.date.issued2017
dc.identifier.issn0169-4332spa
dc.identifier.urihttp://hdl.handle.net/10641/2192
dc.description.abstractThe interaction between surfaces and biological elements, in particular, proteins is critical for the performance of biomaterials and biosensors. This interaction can be controlled by modifying the surface in a process known as biofunctionalization. In this work, the enzyme lactate dehydrogenase (LDH) is used to study the stability of the interaction between a functional protein and amine-functionalized surfaces. Two different functionalization procedures were compared: Activated Vapour Silanization (AVS) and Immersion Silanization (IS). Adsorption kinetics is shown to follow the Langmuir model for AVS-functionalized samples, while IS-functionalized samples show a certain instability if immersed in an aqueous medium for several hours. In turn, the enzymatic activity of LDH is preserved for longer times by using glutaraldehyde as crosslinker between the AVS biofunctional surface and the enzyme.spa
dc.language.isoengspa
dc.publisherApplied Surface Sciencespa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectSurface modificationspa
dc.subjectActivated vapor silanizationspa
dc.subjectInfrared spectroscopyspa
dc.subjectProtein adsorptionspa
dc.subjectLangmuir modelspa
dc.titleStability and activity of lactate dehydrogenase on biofunctional layers deposited by activated vapour silanization (AVS) and immersion silanization (IS).spa
dc.typearticlespa
dc.description.versionpre-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent966 KBspa
dc.identifier.doi10.1016/j.apsusc.2017.04.123spa
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0169433217311509spa


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España