Show simple item record

dc.contributor.authorAlarcón, Sebastián
dc.contributor.authorToro, María de los Ángeles
dc.contributor.authorVillarreal, Carolina
dc.contributor.authorMelo, Rómulo
dc.contributor.authorFernández, Rodrigo
dc.contributor.authorAyuso Sacido, Ángel 
dc.contributor.authorUribe, Daniel
dc.contributor.authorSan Martín, Rody
dc.contributor.authorQuezada, Claudia
dc.date.accessioned2021-09-02T08:56:29Z
dc.date.available2021-09-02T08:56:29Z
dc.date.issued2020
dc.identifier.issn2073-4409spa
dc.identifier.urihttp://hdl.handle.net/10641/2417
dc.description.abstractGlioblastoma multiforme is one of the most malignant types of cancer. This is mainly due to a cell subpopulation with an extremely aggressive potential, called glioblastoma stem-like cells (GSCs). These cells produce high levels of extracellular adenosine which has been associated with increased chemoresistance, migration, and invasion in glioblastoma. In this study, we attempted to elucidate the mechanisms that control extracellular adenosine levels in GSC subtypes. By using primary and U87MG-derived GSCs, we associated increased extracellular adenosine with the mesenchymal phenotype. [3H]-adenosine uptake occurred mainly through the equilibrative nucleoside transporters (ENTs) in GSCs, but mesenchymal GSCs have lower expression and ENT1-mediated uptake activity than proneural GSCs. By analyzing expression and enzymatic activity, we determined that ecto-5′-nucleotidase (CD73) is predominantly expressed in proneural GSCs, driving AMPase activity. While in mesenchymal GSCs, both CD73 and Prostatic Acid Phosphatase (PAP) contribute to the AMP (adenosine monophosphate) hydrolysis. We did not observe significant differences between the expression of proteins involved in the metabolization of adenosine among the GCSs subtypes. In conclusion, the lower expression and activity of the ENT1 transporter in mesenchymal GSCs contributes to the high level of extracellular adenosine that these GSCs present.spa
dc.language.isoengspa
dc.publisherCellsspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectAdenosinespa
dc.subjectGlioblastomaspa
dc.subjectGlioblastoma stem-like cells (GSCs)spa
dc.subjectEquilibrative nucleoside transporter 1 (ENT1)spa
dc.titleDecreased Equilibrative Nucleoside Transporter 1 (ENT1) Activity Contributes to the High Extracellular Adenosine Levels in Mesenchymal Glioblastoma Stem-Like Cells.spa
dc.typearticlespa
dc.description.versionpost-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent2,37 MBspa
dc.identifier.doi10.3390/cells9081914spa
dc.relation.publisherversionhttps://www.mdpi.com/2073-4409/9/8/1914spa


Files in this item

FilesSizeFormatView
cells-09-01914.pdf2.377MbPDFView/Open

This item appears in the following Collection(s)

Show simple item record

Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España