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dc.contributor.authorRipoll Morales, Vanessa
dc.contributor.authorLadero, Miguel
dc.contributor.authorSantos, Victoria E.
dc.date.accessioned2021-10-28T10:52:59Z
dc.date.available2021-10-28T10:52:59Z
dc.date.issued2021
dc.identifier.issn1369-703Xspa
dc.identifier.urihttp://hdl.handle.net/10641/2539
dc.description.abstractBiodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a novel biocatalyst composed by R. terrigena resting cells was evaluated for the first time. Fluid dynamic conditions has been optimized to maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding the effect of initial glycerol concentration in batch runs, no substrate inhibition was detected in the studied conditions (concentrations between 45 and 250 g/L were used). Employing pure glycerol as carbon source, 82.0 g/L 2,3-butanediol titre was achieved, whereas 76.5 g/L were reached using raw glycerol. These numbers involve an achieved yield respect to maximal theoretic yield of 79% and 77%, respectively. A successful kinetic modelling of the bioprocess was developed and it is able to describe both the evolution of concentrations of relevant components with time and the rates calculated at experimental time values. Estimated specific 2,3-butanediol production rate was 0.034 g2,3-BDO/gX⋅h, while the estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol was 2.52 gGly/g2,3-BDO. The obtained results showed that R. terrigena resting cells is a promising biocatalyst, which provides new opportunities for developing and scaling-up glycerol biorefineries.spa
dc.language.isoengspa
dc.publisherBiochemical Engineering Journalspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subject2,3-butanediolspa
dc.subjectResting cellsspa
dc.subjectRaw glycerolspa
dc.subjectKinetic modellingspa
dc.titleKinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.spa
dc.typearticlespa
dc.description.versionpost-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent856 KBspa
dc.identifier.doi10.1016/j.bej.2021.108185spa
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1369703X21002618?via%3Dihubspa


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España