Show simple item record

dc.contributor.authorFahd Qadir, Mirza Muhammad
dc.contributor.authorÁlvarez-Cubela, Silvia
dc.contributor.authorWeitz, Jonathan
dc.contributor.authorK. Panzer, Julia
dc.contributor.authorMoreno-Hernández, Yaisa B.
dc.contributor.authorDomínguez-Bendala, Juan
dc.date.accessioned2021-10-28T11:03:09Z
dc.date.available2021-10-28T11:03:09Z
dc.date.issued2020
dc.identifier.issn2041-1723spa
dc.identifier.urihttp://hdl.handle.net/10641/2541
dc.description.abstractThe culture of live pancreatic tissue slices is a powerful tool for the interrogation of physiology and pathology in an in vitro setting that retains near-intact cytoarchitecture. However, current culture conditions for human pancreatic slices (HPSs) have only been tested for short-term applications, which are not permissive for the long-term, longitudinal study of pancreatic endocrine regeneration. Using a culture system designed to mimic the physiological oxygenation of the pancreas, we demonstrate high viability and preserved endocrine and exocrine function in HPS for at least 10 days after sectioning. This extended lifespan allowed us to dynamically lineage trace and quantify the formation of insulin-producing cells in HPS from both non-diabetic and type 2 diabetic donors. This technology is expected to be of great impact for the conduct of real-time regeneration/developmental studies in the human pancreas.spa
dc.language.isoengspa
dc.publisherNature Communicationsspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleLong-term culture of human pancreatic slices as a model to study real-time islet regeneration.spa
dc.typearticlespa
dc.description.versionpost-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent3.907 KBspa
dc.identifier.doi10.1038/s41467-020-17040-8spa
dc.relation.publisherversionhttps://www.nature.com/articles/s41467-020-17040-8spa


Files in this item

FilesSizeFormatView
s41467-020-17040-8.pdf3.815MbPDFView/Open

This item appears in the following Collection(s)

Show simple item record

Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España