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dc.contributor.authorTurpín, María del Carmen 
dc.contributor.authorPérez Sanz, Fernando
dc.contributor.authorGarcía Solano, José
dc.contributor.authorSebastián León, Patricia
dc.contributor.authorTrujillo Santos, J.
dc.contributor.authorCarbonell, Pablo
dc.contributor.authorEstrada, Eduardo
dc.contributor.authorTuomisto, Anne
dc.contributor.authorHerruzo Priego, Irene 
dc.contributor.authorFennell, Lochlan J.
dc.contributor.authorMäkinen, Markus J.
dc.contributor.authorRodríguez Braun, Edith
dc.contributor.authorJ. Whitehall, Vicki L.
dc.contributor.authorConesa, Ana
dc.contributor.authorConesa Zamora, Pablo
dc.description.abstractBackground. The typical methylation patterns associated with cancer are hypermethylation at gene promoters and global genome hypomethylation. Aberrant CpG island hypermethylation at promoter regions and global genome hypomethylation have not been associated with histological colorectal carcinomas (CRC) subsets. Using Illumina’s 450 k Infinium Human Methylation beadchip, the methylome of 82 CRCs were analyzed, comprising different histological subtypes: 40 serrated adenocarcinomas (SAC), 32 conventional carcinomas (CC) and 10 CRCs showing histological and molecular features of microsatellite instability (hmMSI‐H), and, additionally, 35 normal adjacent mucosae. Scores reflecting the overall methylation at 250 bp, 1 kb and 2 kb from the transcription starting site (TSS) were studied. Results. SAC has an intermediate methylation pattern between CC and hmMSI‐H for the three genome locations. In addition, the shift from promoter hypermethylation to genomic hypomethylation occurs at a small sequence between 250 bp and 1 Kb from the gene TSS, and an asymmetric distribution of methylation was observed between both sides of the CpG islands (N vs. S shores). Conclusion. These findings show that different histological subtypes of CRC have a particular global methylation pattern depending on sequence distance to TSS and highlight the so far underestimated importance of CpGs aberrantly hypomethylated in the clinical phenotype of
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subjectColorectal carcinomaspa
dc.subjectSerrated pathwayspa
dc.subjectMicrosatellite instabilityspa
dc.subjectSerrated adenocarcinomaspa
dc.subjectDNA methylationspa
dc.subjectEpigenetics of carcinogenesisspa
dc.subjectConventional carcinomaspa
dc.subjectMethylation scorespa
dc.titleGlobal Methylome Scores Correlate with Histological Subtypes of Colorectal Carcinoma and Show Different Associations with Common Clinical and Molecular
dc.typejournal articlespa
dc.rights.accessRightsopen accessspa
dc.description.extent672 KBspa
dc.identifier.doi10.3390/cancers 13205165spa

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