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dc.contributor.authorPérez, Elena
dc.contributor.authorMartínez Relimpio, Ana María 
dc.contributor.authorTeijón, César
dc.contributor.authorOlmo, Rosa
dc.contributor.authorTeijón, José María
dc.contributor.authorBlanco, María Dolores
dc.date.accessioned2021-11-12T08:37:23Z
dc.date.available2021-11-12T08:37:23Z
dc.date.issued2015
dc.identifier.issn0378-5173spa
dc.identifier.urihttp://hdl.handle.net/10641/2605
dc.description.abstractMost antitumor drugs usually affect not only rapidly dividing cells, such as those in tumors, but also highly proliferative cells in normal tissues. This nonspecific drawback could be successfully solved by using nanocarriers as controlled drug delivery systems. In this work, pH and redox-responsive nanohydrogels (NG) based on N-isopropylacrilamide (NIPA), N-hydroxyethyl acrylamide (HEEA) 2-acrylamidoethyl carbamate (2AAECM) and N,N′-cystaminebisacrylamide (CBA) as crosslinker were evaluated as bioreducible paclitaxel (PTX) nanocarriers for improving the accumulation of the drug within the tumor tissue and avoiding its conventional side effects. A single dose of PTX solution, unloaded-NHA 80/15/5CBA NG and PTX-loaded NHA 80/15/5-CBA NG (30 mg/kg PTX equivalent) were subcutaneously injected in female athymic nude mice bearing HeLa human tumor xenografts. PTX-loaded nanohydrogels showed higher antitumor activity than free PTX, as tumor evolution and Ki67 detection demonstrated. Histological tumor images revealed a higher content of defective mitotic figures and apoptotic bodies in PTX- treated tumors than in control or unloaded NG treated tumor samples. Nanohydrogels injection did not change any biochemical blood parameters, which means no liver or kidney damage after NG injection. However, differences in antioxidant defenses in MPS systems (liver, kidney and spleen) were observed among treatments, which may indicate an oxidative stress response after PTX injection.spa
dc.language.isoengspa
dc.publisherInternational Journal of Pharmaceuticsspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectAnti-tumor efficacyspa
dc.subjectIn vivo toxicityspa
dc.subjectPaclitaxelspa
dc.subjectStimuli responsivespa
dc.subjectHistopathologyspa
dc.subjectOxidative stressspa
dc.titleImproved antitumor effect of paclitaxel administered in vivo as pH and glutathione-sensitive nanohydrogels.spa
dc.typearticlespa
dc.description.versionpre-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent2904 KBspa
dc.identifier.doi10.1016/j.ijpharm.2015.07.003spa
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0378517315300247?via%3Dihubspa


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España