dc.contributor.author | Duraj, Tomás | |
dc.contributor.author | Carrión Navarro, Josefa | |
dc.contributor.author | Seyfried, Thomas N. | |
dc.contributor.author | García Romero, Noemí | |
dc.contributor.author | Ayuso Sacido, Ángel | |
dc.date.accessioned | 2022-01-13T12:47:13Z | |
dc.date.available | 2022-01-13T12:47:13Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 2212-8778 | spa |
dc.identifier.uri | http://hdl.handle.net/10641/2659 | |
dc.description.abstract | Background
Aberrant metabolism is recognized as a hallmark of cancer, a pillar necessary for cellular proliferation. Regarding bioenergetics (ATP generation), most cancers display a preference not only toward aerobic glycolysis (“Warburg effect”) and glutaminolysis (mitochondrial substrate level-phosphorylation) but also toward other metabolites such as lactate, pyruvate, and fat-derived sources. These secondary metabolites can assist in proliferation but cannot fully cover ATP demands.
Scope of review
The concept of a static metabolic profile is challenged by instances of heterogeneity and flexibility to meet fuel/anaplerotic demands. Although metabolic therapies are a promising tool to improve therapeutic outcomes, either via pharmacological targets or press-pulse interventions, metabolic plasticity is rarely considered. Lack of bioenergetic analysis in vitro and patient-derived models is hindering translational potential. Here, we review the bioenergetics of cancer and propose a simple analysis of major metabolic pathways, encompassing both affordable and advanced techniques. A comprehensive compendium of Seahorse XF bioenergetic measurements is presented for the first time.
Major conclusions
Standardization of principal readouts might help researchers to collect a complete metabolic picture of cancer using the most appropriate methods depending on the sample of interest. | spa |
dc.language.iso | eng | spa |
dc.publisher | Molecular Metabolism | spa |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Cancer | spa |
dc.subject | Energy metabolism | spa |
dc.subject | Glycolysis | spa |
dc.subject | Oxidative phosphorylation | spa |
dc.subject | Research design | spa |
dc.title | Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle. | spa |
dc.type | journal article | spa |
dc.type.hasVersion | AM | spa |
dc.rights.accessRights | open access | spa |
dc.description.extent | 3250 KB | spa |
dc.identifier.doi | 10.1016/j.molmet.2021.101389 | spa |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S2212877821002404?via%3Dihub | spa |