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dc.contributor.authorDuraj, Tomás
dc.contributor.authorCarrión Navarro, Josefa
dc.contributor.authorSeyfried, Thomas N.
dc.contributor.authorGarcía Romero, Noemí 
dc.contributor.authorAyuso Sacido, Ángel
dc.description.abstractBackground Aberrant metabolism is recognized as a hallmark of cancer, a pillar necessary for cellular proliferation. Regarding bioenergetics (ATP generation), most cancers display a preference not only toward aerobic glycolysis (“Warburg effect”) and glutaminolysis (mitochondrial substrate level-phosphorylation) but also toward other metabolites such as lactate, pyruvate, and fat-derived sources. These secondary metabolites can assist in proliferation but cannot fully cover ATP demands. Scope of review The concept of a static metabolic profile is challenged by instances of heterogeneity and flexibility to meet fuel/anaplerotic demands. Although metabolic therapies are a promising tool to improve therapeutic outcomes, either via pharmacological targets or press-pulse interventions, metabolic plasticity is rarely considered. Lack of bioenergetic analysis in vitro and patient-derived models is hindering translational potential. Here, we review the bioenergetics of cancer and propose a simple analysis of major metabolic pathways, encompassing both affordable and advanced techniques. A comprehensive compendium of Seahorse XF bioenergetic measurements is presented for the first time. Major conclusions Standardization of principal readouts might help researchers to collect a complete metabolic picture of cancer using the most appropriate methods depending on the sample of
dc.publisherMolecular Metabolismspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subjectEnergy metabolismspa
dc.subjectOxidative phosphorylationspa
dc.subjectResearch designspa
dc.titleMetabolic therapy and bioenergetic analysis: The missing piece of the
dc.typejournal articlespa
dc.rights.accessRightsopen accessspa
dc.description.extent3250 KBspa

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Atribución-NoComercial-SinDerivadas 3.0 España
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