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dc.contributor.authorSantibáñez, Juan F.
dc.contributor.authorFrontelo, Pilar
dc.contributor.authorMartínez, Jorge
dc.contributor.authorQuintanilla, Miguel
dc.contributor.authorIglesias Badiola, Maite 
dc.date.accessioned2012-08-09T03:05:23Z
dc.date.available2012-08-09T03:05:23Z
dc.date.issued2000-05-23
dc.identifier.issn0006-291X
dc.identifier.urihttp://hdl.handle.net/10641/281
dc.description.abstractTransformed PDV keratonocytes respond to TGF-β1 by stimulating cell motility and invasiveness concomitantly to enhancement of the urokinae-type plasmiogen activator (uPA) expression/secretion. Depletion of extracellular signal-regulated kinase (EREK1,2) proteins by treatment of PDV cells with antinese oligonucleotides reduced basal uPA production and abolished stimulation of uPA secreted levels and cell motility by TGF-β1. PD098059, an inhibitor of mitogenactivated protein kinase (MAPK) kinase (MEK), decreased TGF-β1-induced uPA mRNA expression, secreted activity in a dose-dependent manner, and abrogated TGF-β1-stimulated cell motility and invasiveness. PDV-derived dominant-negative RasN17 cell transfectants secreted similar amounts of uPA and exhibited similar invasive abilities as the parental cells or control clones, but were unable to respond to TGF-β1 for stimulation of uPA-secreted levels and invasiveness. These results suggest that a Ras/MAPK transduction pathway is involved in the invasive response ot transformed keratinocytes to TGF-β1.spa
dc.language.isoengspa
dc.publisherAcademic Pressspa
dc.relation.ispartofseriesBiochemical and Biophysical Research Communications;273spa
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectTGF-β1spa
dc.subjectuPAspa
dc.subjectKeratinocytesspa
dc.subjectRasspa
dc.subjectMEKspa
dc.subjectERKspa
dc.subjectInvasivenessspa
dc.titleInvolvement of the Ras/MAPK Signalling Pathway in the Modulation of Urokinase Production and Cellular Invasiveness by Transforming Growth Factor TGF-β1 in Transformed Keratinocytesspa
dc.typearticlespa


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