dc.contributor.author | Santibáñez, Juan F. | |
dc.contributor.author | Frontelo, Pilar | |
dc.contributor.author | Martínez, Jorge | |
dc.contributor.author | Quintanilla, Miguel | |
dc.contributor.author | Iglesias Badiola, Maite | |
dc.date.accessioned | 2012-08-09T03:05:23Z | |
dc.date.available | 2012-08-09T03:05:23Z | |
dc.date.issued | 2000-05-23 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.uri | http://hdl.handle.net/10641/281 | |
dc.description.abstract | Transformed PDV keratonocytes respond to TGF-β1 by stimulating cell motility and invasiveness concomitantly to enhancement of the urokinae-type plasmiogen activator (uPA) expression/secretion. Depletion of extracellular signal-regulated kinase (EREK1,2) proteins by treatment of PDV cells with antinese oligonucleotides reduced basal uPA production and abolished stimulation of uPA secreted levels and cell motility by TGF-β1. PD098059, an inhibitor of mitogenactivated protein kinase (MAPK) kinase (MEK), decreased TGF-β1-induced uPA mRNA expression, secreted activity in a dose-dependent manner, and abrogated TGF-β1-stimulated cell motility and invasiveness. PDV-derived dominant-negative RasN17 cell transfectants secreted similar amounts of uPA and exhibited similar invasive abilities as the parental cells or control clones, but were unable to respond to TGF-β1 for stimulation of uPA-secreted levels and invasiveness. These results suggest that a Ras/MAPK transduction pathway is involved in the invasive response ot transformed keratinocytes to TGF-β1. | spa |
dc.language.iso | eng | spa |
dc.publisher | Academic Press | spa |
dc.relation.ispartofseries | Biochemical and Biophysical Research Communications;273 | spa |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | TGF-β1 | spa |
dc.subject | uPA | spa |
dc.subject | Keratinocytes | spa |
dc.subject | Ras | spa |
dc.subject | MEK | spa |
dc.subject | ERK | spa |
dc.subject | Invasiveness | spa |
dc.title | Involvement of the Ras/MAPK Signalling Pathway in the Modulation of Urokinase Production and Cellular Invasiveness by Transforming Growth Factor TGF-β1 in Transformed Keratinocytes | spa |
dc.type | journal article | spa |