dc.contributor.author | Jackson, Sarah E. | |
dc.contributor.author | Chen, Kevin C. | |
dc.contributor.author | Groves, Ian J. | |
dc.contributor.author | Sedikides, George X. | |
dc.contributor.author | Gandhi, Amar | |
dc.contributor.author | Houldcroft, Charlotte J. | |
dc.contributor.author | Poole, Emma L. | |
dc.contributor.author | Montanuy, Inmaculada | |
dc.contributor.author | Mason, Gavin M. | |
dc.contributor.author | Okecha, Georgina | |
dc.contributor.author | Reeves, Matthew B. | |
dc.contributor.author | Sinclair, John H. | |
dc.contributor.author | Wills, Mark R. | |
dc.date.accessioned | 2022-02-16T12:45:46Z | |
dc.date.available | 2022-02-16T12:45:46Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 1664-3224 | spa |
dc.identifier.uri | http://hdl.handle.net/10641/2825 | |
dc.description.abstract | Human cytomegalovirus (HCMV) infection is not cleared by the initial immune response but persists for the lifetime of the host, in part due to its ability to establish a latent infection in cells of the myeloid lineage. HCMV has been shown to manipulate the secretion of cellular proteins during both lytic and latent infection; with changes caused by latent infection mainly investigated in CD34+ progenitor cells. Whilst CD34+ cells are generally bone marrow resident, their derivative CD14+ monocytes migrate to the periphery where they briefly circulate until extravasation into tissue sites. We have analyzed the effect of HCMV latent infection on the secretome of CD14+ monocytes, identifying an upregulation of both CCL8 and CXCL10 chemokines in the CD14+ latency-associated secretome. Unlike CD34+ cells, the CD14+ latency-associated secretome did not induce migration of resting immune cell subsets but did induce migration of activated NK and T cells expressing CXCR3 in a CXCL10 dependent manner. As reported in CD34+ latent infection, the CD14+ latency-associated secretome also suppressed the anti-viral activity of stimulated CD4+ T cells. Surprisingly, however, co-culture of activated autologous CD4+ T cells with latently infected monocytes resulted in reactivation of HCMV at levels comparable to those observed using M-CSF and IL-1β cytokines. We propose that these events represent a potential strategy to enable HCMV reactivation and local dissemination of the virus at peripheral tissue sites. | spa |
dc.language.iso | eng | spa |
dc.publisher | Frontiers in Immunology | spa |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Human cytomegalovirus | spa |
dc.subject | Latency | spa |
dc.subject | Monocytes | spa |
dc.subject | Reactivation | spa |
dc.subject | CD4+ T cells | spa |
dc.title | Latent Cytomegalovirus-Driven Recruitment of Activated CD4+ T Cells Promotes Virus Reactivation. | spa |
dc.type | journal article | spa |
dc.type.hasVersion | AM | spa |
dc.rights.accessRights | open access | spa |
dc.description.extent | 2637 KB | spa |
dc.identifier.doi | 10.3389/fimmu.2021.657945 | spa |
dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fimmu.2021.657945/full | spa |