Transient accumulation and bidirectional movement of KIF13B in primary cilia.
Author: Dupont Juhl, Alice; Anvarian, Zeinab; Kuhns, Stefanie; Berges, Julia; Andersen, Jens S.; Wüstner, Daniel; B. Pedersen, Lotte
Abstract: Primary cilia are microtubule-based sensory organelles
whose assembly and function rely on the conserved bidirectional
intraflagellar transport (IFT) system, which is powered by anterograde
kinesin-2 and retrograde cytoplasmic dynein-2 motors. Nematodes
additionally employ a cell-type-specific kinesin-3 motor, KLP-6,
which moves within cilia independently of IFT and regulates ciliary
content and function. Here, we provide evidence that a KLP-6
homolog, KIF13B, undergoes bursts of bidirectional movement
within primary cilia of cultured immortalized human retinal
pigment epithelial (hTERT-RPE1) cells. Anterograde and retrograde
intraciliary velocities of KIF13B were similar to those of IFT
(as assayed using IFT172–eGFP), but intraciliary movement of
KIF13B required its own motor domain and appeared to be cell-type
specific. Our work provides the first demonstration of motor-driven,
intraciliary movement by a vertebrate kinesin other than kinesin-2
motors.
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