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Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization.

dc.contributor.authorLópez-Sanz, Celia
dc.contributor.authorSánchez Martínez, Elisa
dc.contributor.authorJiménez Sáiz, Rodrigo
dc.date.accessioned2023-04-27T09:28:06Z
dc.date.available2023-04-27T09:28:06Z
dc.date.issued2022
dc.identifier.issn2666-1667spa
dc.identifier.urihttps://hdl.handle.net/10641/3362
dc.description.abstractIn this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and b-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches.spa
dc.language.isoengspa
dc.publisherSTAR Protocolsspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectCell biologyspa
dc.subjectCell culturespa
dc.subjectFlow cytometry/Mass cytometryspa
dc.subjectHealth sciencesspa
dc.subjectImmunologyspa
dc.subjectModel organismsspa
dc.titleProtocol to desensitize human and murine mast cells after polyclonal IgE sensitization.spa
dc.typejournal articlespa
dc.type.hasVersionAMspa
dc.rights.accessRightsopen accessspa
dc.description.extent3350 KBspa
dc.identifier.doi10.1016/j.xpro.2022.101755spa
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2666166722006359spa


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Atribución-NoComercial-SinDerivadas 3.0 España
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