Differences in the degree of inhibition of NDP reductase by chemical inactivation and by the thermosensitive mutation nrdA101 in Escherichia coli suggest an effect on chromosome segregation.
Resumen: NDP reductase activity can be inhibited either by treatment with
hydroxyurea or by incubation of an nrdAts mutant strain at the non-permissive
temperature. Both methods inhibit replication, but experiments on these two
types of inhibition yielded very different results. The chemical treatment
immediately inhibited DNA synthesis but did not affect the cell and nucleoid
appearance, while the incubation of an nrdA101 mutant strain at the nonpermissive
temperature inhibited DNA synthesis after more than 50 min, and
resulted in aberrant chromosome segregation, long filaments, and a high
frequency of anucleate cells. These phenotypes are not induced by SOS. In view
of these results, we suggest there is an indirect relationship between NDP
reductase and the chromosome segregation machinery through the maintenance
of the proposed replication hyperstructure.
Identificador universal: https://hdl.handle.net/10641/3558
Fecha: 2006
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