Synergistic Combination of Antimicrobial Peptides and Cationic Polyitaconates in Multifunctional PLA Fibers.
Autor: Chiloeches, Alberto; Zágora, Jakub; Plachá, Daniela; Torres, Marcelo D. T.; De la Fuente-Núñez, Cesar; López Fabal, Fátima; Gil-Romero, Yolanda; Fernández-García, Raquel; Fernández-García, Marta; Echeverría, Coro; Muñoz-Bonilla, Alexandra
Resumen: Combining different antimicrobial agents has
emerged as a promising strategy to enhance efficacy and address
resistance evolution. In this study, we investigated the synergistic
antimicrobial effect of a cationic biobased polymer and the
antimicrobial peptide (AMP) temporin L, with the goal of
developing multifunctional electrospun fibers for potential
biomedical applications, particularly in wound dressing. A clickable
polymer with pendent alkyne groups was synthesized by using a
biobased itaconic acid building block. Subsequently, the polymer
was functionalized through click chemistry with thiazolium groups
derived from vitamin B1 (PTTIQ), as well as a combination of
thiazolium and AMP temporin L, resulting in a conjugate
polymer−peptide (PTTIQ-AMP). The individual and combined
effects of the cationic PTTIQ, Temporin L, and PTTIQ-AMP were evaluated against Gram-positive and Gram-negative bacteria as
well as Candida species. The results demonstrated that most combinations exhibited an indifferent effect, whereas the covalently
conjugated PTTIQ-AMP displayed an antagonistic effect, potentially attributed to the aggregation process. Both antimicrobial
compounds, PTTIQ and temporin L, were incorporated into poly(lactic acid) electrospun fibers using the supercritical solvent
impregnation method. This approach yielded fibers with improved antibacterial performance, as a result of the potent activity
exerted by the AMP and the nonleaching nature of the cationic polymer, thereby enhancing long-term effectiveness.
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