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dc.contributor.authorRynne-Vidal, Angela
dc.contributor.authorAu-Yeung, Chi Lam
dc.contributor.authorJiménez-Heffernan, José A.
dc.contributor.authorPérez-Lozano, María Luisa
dc.contributor.authorCremades-Jimeno, Lucía
dc.contributor.authorBárcena, Carmen
dc.contributor.authorCristóbal García, Ignacio 
dc.contributor.authorFernández-Chacón, Concepción
dc.contributor.authorYeung, Tsz Lun
dc.contributor.authorC Mok, Samuel
dc.contributor.authorSandoval, Pilar
dc.contributor.authorLópez Cabrera, Manuel
dc.date.accessioned2017-10-11T10:26:46Z
dc.date.available2017-10-11T10:26:46Z
dc.date.issued2017
dc.identifier.issn1096-9896
dc.identifier.urihttp://hdl.handle.net/10641/1355
dc.description.abstractPeritoneal dissemination is the primary metastatic route of ovarian cancer (OvCa), and is often accompanied by the accumulation of ascitic fluid. The peritoneal cavity is lined by mesothelial cells (MCs), which can be converted into carcinoma-associated fibroblasts (CAFs) through mesothelial-to-mesenchymal transition (MMT). Here, we demonstrate that MCs isolated from ascitic fluid (AFMCs) of OvCa patients with peritoneal implants also undergo MMT and promote subcutaneous tumour growth in mice. RNA sequencing of AFMCs revealed that MMT-related pathways – including transforming growth factor (TGF)- signalling – are differentially regulated, and a gene signature was verified in peritoneal implants from OvCa patients. In a mouse model, pre-induction of MMT resulted in increased peritoneal tumour growth, whereas interfering with the TGF- receptor reduced metastasis. MC-derived CAFs showed activation of Smad-dependent TGF- signalling, which was disrupted in OvCa cells, despite their elevated TGF- production. Accordingly, targeting Smad-dependent signalling in the peritoneal pre-metastatic niche in mice reduced tumour colonization, suggesting that Smad-dependent MMT could be crucial in peritoneal carcinomatosis. Together, these results indicate that bidirectional communication between OvCa cells and MC-derived CAFs, via TGF--mediated MMT, seems to be crucial to form a suitable metastatic niche. We suggest MMT as a possible target for therapeutic intervention and a potential source of biomarkers for improving OvCa diagnosis and/or prognosis.spa
dc.language.isoengspa
dc.publisherJournal of Pathologyspa
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectPeritoneal metastasisspa
dc.subjectOvarian cancerspa
dc.subjectCarcinoma-associated fibroblastsspa
dc.subjectMesothelial-to-mesenchymal transitionspa
dc.subjectAscitesspa
dc.titleMesothelial-to-mesenchymal transition as a possible therapeutic target in peritoneal metastasis of ovarian cancer.spa
dc.typearticlespa
dc.description.versionpost-printspa
dc.rights.accessRightsopenAccessspa
dc.description.extent2310 KBspa


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