Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes.
Abstract: Titanium dioxide nanoparticles (TiO2-NPs) are being used in several consumer products. The high refractive
index of nano-scaled titanium dioxide particles allows them to protect from UV radiation, and so, they can be
found as one of the main components of cosmetics and suncreens. Many studies have reported the potential
toxicological effects associated to TiO2-NPs such as ROS generation, DNA damage, apoptosis and cell cycle
arrest, among others. The continuous and systematic use of TiO2-NPs in cosmetic products requires a full
comprehension of the risks involving their sustained contact with the human skin. Thus, it is important to
evaluate not only the hazardous effects but to elucidate the biomolecular mechanisms involved in such effects.
Based on this premises, we have evaluated the potential toxicity of TiO2-NPs using a human epithelial cell
culture (HaCaT cells) as in-vitro model, together with different bioanalytical approaches and mass spectrometrybased quantitative proteomics, to gain a deeper insight into the molecular mechanisms of toxicity associated to
TiO2-NPs exposure
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