dc.contributor.author | Füreder, Vera | |
dc.contributor.author | Rodríguez-Colinas, Bárbara | |
dc.contributor.author | Cervantes, Fadia V. | |
dc.contributor.author | Fernández-Arrojo, Lucía | |
dc.contributor.author | Poveda, Ana | |
dc.contributor.author | Jiménez-Barbero, Jesús | |
dc.contributor.author | Ballesteros, Antonio O. | |
dc.contributor.author | Plou, Francisco J. | |
dc.date.accessioned | 2021-12-16T08:54:07Z | |
dc.date.available | 2021-12-16T08:54:07Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0021-8561 | spa |
dc.identifier.uri | http://hdl.handle.net/10641/2623 | |
dc.description.abstract | The transglycosylation activity of a novel commercial β-galactosidase from
Bifidobacterium bifidum (Saphera) was evaluated. The optimal conditions of operation of
this enzyme, measured with o-nitrophenyl-β-D-galactopyranoside, were 40 °C and pH
around 6.0. Although at low lactose concentrations the character of this enzyme was
basically hydrolytic, an increase of lactose concentration to 400 g/L resulted in a
significant formation (107.2 g/L, 27% yield) of prebiotic galactooligosaccharides (GOS).
The maximum amount of GOS was obtained at a lactose conversion of approximately 90%,
which contrasts with other β-galactosidases, for which the highest GOS yield is achieved
at 40-50% lactose conversion. Using HPAEC-PAD, semipreparative HPLC-HILIC, MS, 1D
and 2D NMR, we determined the structure of most of the GOS synthesized by this enzyme.
The main identified products were Gal-β(13)-Gal-β(14)-Glc (3´-O-Beta-galactosyllactose), Gal-β(1→6)-Glc (allolactose), Gal-β(13)-Glc (3-galactosyl-glucose), Galβ(1→3)-Gal (3-galactobiose) and the tetrasaccharide Gal-β(13)-Gal-β(13)-Galβ(14)-Glc. In general, the B. bifidum β-galactosidase showed a tendency to form β(13)
linkages followed by β(16), and more scarcely β(14). | spa |
dc.language.iso | eng | spa |
dc.publisher | Journal of Agricultural and Food Chemistry | spa |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Galactooligosaccharides | spa |
dc.subject | Prebiotics | spa |
dc.subject | Transglycosylation | spa |
dc.subject | βGalactosidase | spa |
dc.subject | Bifidobacteria | spa |
dc.subject | Glycosidases | spa |
dc.title | Selective synthesis of galactooligosaccharides containing β(1→3) linkages with Beta-galactosidase from Bifidobacterium bifidum | spa |
dc.type | journal article | spa |
dc.type.hasVersion | SMUR | spa |
dc.rights.accessRights | open access | spa |
dc.description.extent | 1,17 MB | spa |
dc.identifier.doi | https://doi.org/10.1021/acs.jafc.0c00997 | spa |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acs.jafc.0c00997 | spa |