Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization.
Resumen: In this protocol, we provide detailed instructions to desensitize human and murine mast cells
(MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation
assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry
and b-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other
cell types, immunofluorescence, live imaging, and omics approaches.
Identificador universal: https://hdl.handle.net/10641/3362
Fecha: 2022
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Ficheros | Tamaño | Formato | Ver |
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1-s2.0-S2666166722006359-main.pdf | 3.271Mb | Ver/ |
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