Browsing by Author "Bosch, Ricardo J."
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Item Bisfenol A en la insuficiencia renal: ¿hasta cuándo se podrá usar? ¿Es la hora de evitarlo?.(Nefrología, 2024) González-Parra, Emilio; Moreno-Gómez-Toledano, Rafael; Mas-Fontao, Sebastián; Bosch, Ricardo J.Item Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.(https://www.mdpi.com/2218-273X/11/10/1429, 2021) Moreno Gómez Toledano, Rafael; Sánchez Esteban, Sandra; Cook, Alberto; Mínguez Moratinos, Marta; Ramírez Carracedo, Rafael; Reventun, Paula; Delgado Marín, María; Bosch, Ricardo J.; Saura, MartaBisphenol A (BPA) is a widespread endocrine disruptor affecting many organs and systems. Previous work in our laboratory demonstrated that BPA could induce death due to necroptosis in murine aortic endothelial cells (MAECs). This work aims to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells. The β-Gal assays showed interesting differences in cell senescence at relatively low doses (100 nM and 5 µM). Western blots confirmed that proteins involved in senescence mechanisms, p16 and p21, were overexpressed in the presence of BPA. In addition, the UPR (unfolding protein response) system, which is part of the senescent phenotype, was also explored by Western blot and qPCR, confirming the involvement of the PERK-ATF4-CHOP pathway (related to pathological processes). The endothelium of mice treated with BPA showed an evident increase in the expression of the proteins p16, p21, and CHOP, confirming the results observed in cells. Our results demonstrate that oxidative stress induced by BPA leads to UPR activation and senescence since pretreatment with N-acetylcysteine (NAC) in BPA-treated cells reduced the percentage of senescent cells prevented the overexpression of proteins related to BPA-induced senescence and reduced the activation of the UPR system. The results suggest that BPA participates actively in accelerated cell aging mechanisms, affecting the vascular endothelium and promoting cardiovascular diseases.Item New environmental factors related to diabetes risk in humans: Emerging bisphenols used in synthesis of plastics.(World Journal of Diabetes, 2023) Moreno-Gómez-Toledano, Rafael; Delgado-Marín, María; Cook-Calvete, Alberto; González-Cucharero, Claudia; Alcharani, Nunzio; Jiménez-Guirado, Beatriz; Hernandez, Ignacio; Ramírez Carracedo, Rafael; Tesoro Santos, Laura; Botana, Laura; Sánchez-Esteban, Sandra; Diez-Mata, Javier; Zamorano, Jose Luis; Bosch, Ricardo J.; Zaragoza Sánchez, Carlos; Saura Redondo, MartaBACKGROUND Diabetes mellitus (DM) is one of the largest global health emergencies of the 21st century. In recent years, its connection with environmental pollutants, such as bisphenol A (BPA), has been demonstrated; consequently, new structurally similar molecules are used to replace BPA in the plastics industry (BPS, BPF and BPAF). AIM To carry out a systematic review to allow coherent evaluation of the state of the art. Subsequently, a meta-analysis was performed to unify the existing quantitative data. METHODS Firstly, a systematic review was carried out, using the terms “(bisphenol) AND (Diabetes OR Hyperglycemia)”, to maximize the number of results. Subsequently, three authors analyzed the set of articles. Finally, a meta-analysis was performed for each BP, using RevMan software. In addition, funnel plots were developed to study publication bias. RESULTS The systematic analysis of the literature revealed 13 recent articles (2017–2023) related to the study paradigm. The qualitative analysis showed interesting data linking diabetes to the three most widely used substitute BPs in the industry: BPS, BPF and BPAF. Finally, the meta-analysis determined a positive relationship with BPS, BPF and BPAF, which was only statistically significant with BPS. CONCLUSION There is a need to apply the precautionary principle, regulating the use of new BPs. Therefore, replacing BPA with BPS, BPF or BPAF is unlikely to protect the population from potential health risks, such as DM