Protein Kinase C-Gamma Knockout Mice Show Impaired Hippocampal Short-Term Memory While Preserved Long-Term Memory.
dc.contributor.author | Gomis-González, Maria | |
dc.contributor.author | Galera-López, Lorena | |
dc.contributor.author | Ten Blanco, Marc | |
dc.contributor.author | Busquets- Garcia, Arnau | |
dc.contributor.author | Cox, Thomas | |
dc.contributor.author | Maldonado, Rafael | |
dc.contributor.author | Ozaita, Andrés | |
dc.date.accessioned | 2022-05-23T08:38:29Z | |
dc.date.available | 2022-05-23T08:38:29Z | |
dc.date.issued | 2021 | |
dc.description.abstract | The brain encodes, stores, and retrieves relevant information in the form of memories that are classified as short-term (STM) and long-term memories (LTM) depending on the interval between acquisition and retrieval. It is classically accepted that STM undergo a consolidation process to form LTM, but the molecular determinants involved are not well understood. Among the molecular components relevant for memory formation, we focused our attention on the protein kinase C (PKC) family of enzymes since they control key aspects of the synaptic plasticity and memory. Within the different PKC isoforms, PKC-gamma has been specifically associated with learning and memory since mice lacking this isoform (PKC-gamma KO mice) showed mild cognitive impairment and deficits in hippocampal synaptic plasticity. We now reveal that PKC-gamma KO mice present a severe impairment in hippocampal-dependent STM using different memory tests including the novel object-recognition and novel place-recognition, context fear conditioning and trace fear conditioning. In contrast, no differences between genotypes were observed in an amygdala-dependent test, the delay fear conditioning. Strikingly, all LTM tasks that could be assessed 24 h after acquisition were not perturbed in the KO mice. The analysis of c-Fos expression in several brain areas after trace fear conditioning acquisition showed a blunted response in the dentate gyrus of PKC-gamma KO mice compared with WT mice, but such differences between genotypes were absent when the amygdala or the prefrontal cortex were examined. In the hippocampus, PKC-gamma was found to translocate to the membrane after auditory trace, but not after delay fear conditioning. Together, these results indicate that PKC-gamma dysfunction affects specifically hippocampal-dependent STM performance and disclose PKC-gamma as a molecular player differentially involved in STM and LTM processes. | spa |
dc.description.extent | 2109 KB | spa |
dc.identifier.doi | 10.1007/s12035-020-02135-6 | spa |
dc.identifier.issn | 0893-7648 | spa |
dc.identifier.uri | http://hdl.handle.net/10641/2980 | |
dc.language.iso | eng | spa |
dc.publisher | Molecular Neurobiology | spa |
dc.relation.publisherversion | https://link.springer.com/article/10.1007/s12035-020-02135-6 | spa |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.accessRights | open access | spa |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Short-term memory | spa |
dc.subject | Long-term memory | spa |
dc.subject | PKC-gamma | spa |
dc.subject | Memory acquisition | spa |
dc.subject | c-Fos 26 expression | spa |
dc.title | Protein Kinase C-Gamma Knockout Mice Show Impaired Hippocampal Short-Term Memory While Preserved Long-Term Memory. | spa |
dc.type | journal article | spa |
dc.type.hasVersion | SMUR | spa |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 9c7d6236-0c6b-48ca-bfbe-1598a1ac72dd | |
relation.isAuthorOfPublication.latestForDiscovery | 9c7d6236-0c6b-48ca-bfbe-1598a1ac72dd |
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