Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration.
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2021
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Biochemical Engineering Journal
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Abstract
Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple
industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for
the bioprocess under conventional growing conditions. In the present work, a novel biocatalyst composed by
R. terrigena resting cells was evaluated for the first time. Fluid dynamic conditions has been optimized to
maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding the effect of initial
glycerol concentration in batch runs, no substrate inhibition was detected in the studied conditions (concentrations
between 45 and 250 g/L were used). Employing pure glycerol as carbon source, 82.0 g/L 2,3-butanediol
titre was achieved, whereas 76.5 g/L were reached using raw glycerol. These numbers involve an achieved yield
respect to maximal theoretic yield of 79% and 77%, respectively. A successful kinetic modelling of the bioprocess
was developed and it is able to describe both the evolution of concentrations of relevant components with time
and the rates calculated at experimental time values. Estimated specific 2,3-butanediol production rate was
0.034 g2,3-BDO/gX⋅h, while the estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol was
2.52 gGly/g2,3-BDO. The obtained results showed that R. terrigena resting cells is a promising biocatalyst, which
provides new opportunities for developing and scaling-up glycerol biorefineries.
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2,3-butanediol, Resting cells, Raw glycerol, Kinetic modelling