Human Mitochondrial HMG-CoA Synthase Deficiency: Role of Enzyme Dimerization Surface and Characterization of Three New Patients.
dc.contributor.author | Puisac, Beatriz | |
dc.contributor.author | Marcos Alcalde, Íñigo | |
dc.contributor.author | Hernández Marcos, María | |
dc.contributor.author | Tobajas Morlana, Pilar | |
dc.contributor.author | Levtova, Alina | |
dc.contributor.author | C. Schwahn, Bernd | |
dc.contributor.author | DeLaet, Corinne | |
dc.contributor.author | Lace, Baiba | |
dc.contributor.author | Gómez Puertas, Paulino | |
dc.contributor.author | Pié, Juan | |
dc.date.accessioned | 2018-10-15T10:44:17Z | |
dc.date.available | 2018-10-15T10:44:17Z | |
dc.date.issued | 2018 | |
dc.description.abstract | Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase deficiency (mitochondrial HMG-CoA synthase deficiency or mHS deficiency, OMIM #605911) is an inborn error of metabolism that affects ketone body synthesis. Acute episodes include vomiting, lethargy, hepatomegaly, hypoglycemia and dicarboxylic aciduria. The diagnosis is difficult due to the relatively unspecific clinical and biochemical presentation, and fewer than 30 patients have been described. This work describes three new patients with mHS deficiency and two missense mutations c.334C>T (p.R112W) and c.430G>T (p.V144L) previously not reported. We developed a new method to express and measure the activity of the enzyme and in this work the study is extended to ten new missense variants including those of our patients. Enzymatic assays showed that three of the mutant proteins retained some but seven completely lacked activity. The identification of a patient homozygous for a mutation that retains 70% of enzyme activity opens the door to a new interpretation of the disease by demonstrating that a modest impairment of enzyme function can actually produce symptoms. This is also the first study employing molecular dynamics modelling of the enzyme mutations. We show that the correct maintenance of the dimerization surface is crucial for retaining the structure of the active center and therefore the activity of the enzyme. | spa |
dc.description.extent | 1746 KB | spa |
dc.identifier.doi | 10.3390/ijms19041010 | |
dc.identifier.issn | 1422-0067 | spa |
dc.identifier.uri | http://hdl.handle.net/10641/1506 | |
dc.language.iso | eng | spa |
dc.publisher | International Journal of Molecular Sciences | spa |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.accessRights | open access | spa |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Mitochondrial HMG-CoA synthase | spa |
dc.subject | Enzyme dimerization | spa |
dc.subject | Ketone bodies | spa |
dc.title | Human Mitochondrial HMG-CoA Synthase Deficiency: Role of Enzyme Dimerization Surface and Characterization of Three New Patients. | spa |
dc.type | journal article | spa |
dc.type.hasVersion | AM | spa |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 699d5be0-fd5f-4819-99da-c5057d24c3fc | |
relation.isAuthorOfPublication.latestForDiscovery | 699d5be0-fd5f-4819-99da-c5057d24c3fc |
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