López-Sanz, CeliaSánchez Martínez, ElisaJiménez Sáiz, Rodrigo2023-04-272023-04-2720222666-1667https://hdl.handle.net/10641/3362In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and b-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches.engAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/Cell biologyCell cultureFlow cytometry/Mass cytometryHealth sciencesImmunologyModel organismsjournal articleopen access10.1016/j.xpro.2022.101755