Cytotoxicity Assessment of a New Design for a Biodegradable Ureteral Mitomycin Drug-Eluting Stent in Urothelial Carcinoma Cell Culture.
Autor: Soria, Federico; Martínez-Pla, Luna; Aznar-Cervantes, Salvador D.; De la Cruz, Julia E.; Fernández, Tomás; Pérez-Fentes, Daniel; Llanes González, Luis; Sánchez-Margallo, Francisco Miguel
Resumen: Urothelial tumour of the upper urinary tract is a rare neoplasm, but unfortunately, it
has a high recurrence rate. The reduction of these tumour recurrences could be achieved by the
intracavitary instillation of adjuvant chemotherapy after nephron-sparing treatment in selected
patients, but current instillation methods are ineffective. Therefore, the aim of this in vitro study is
to evaluate the cytotoxic capacity of a new instillation technology through a biodegradable ureteral
stent/scaffold coated with a silk fibroin matrix for the controlled release of mitomycin C as an
anti-cancer drug. Through a comparative study, we assessed, in urothelial carcinoma cells in a human
cancer T24 cell culture for 3 and 6 h, the cytotoxic capacity of mitomycin C by viability assay using
the CCK-8 test (Cell counting Kit-8). Cell viability studies in the urothelial carcinoma cell line confirm
that mitomycin C embedded in the polymeric matrix does not alter its cytotoxic properties and causes
a significant decrease in cell viability at 6 h versus in the control groups. These findings have a clear
biomedical application and could be of great use to decrease the recurrence rate in patients with
upper tract urothelial carcinomas by increasing the dwell time of anti-cancer drugs.
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