Ontogeny of ATP hydrolysis and isoform expression of the Plasma Membrane Ca2+-ATPase in mouse brain.
Abstract: Background: Plasma membrane Ca2+-ATPases (PMCAs) are high affinity Ca2+ transporters
actively involved in intracellular Ca2+ homeostasis. Considering the critical role of Ca2+ signalling in
neuronal development and plasticity, we have analyzed PMCA-mediated Ca2+-ATPase activity and
PMCA-isoform content in membranes from mouse cortex, hippocampus and cerebellum during
postnatal development.
Results: PMCA activity was detected from birth, with a faster evolution in cortex than in
hippocampus and cerebellum. Western blots revealed the presence of the four isoforms in all
regions, with similar increase in their expression patterns as those seen for the activity profile.
Immunohistochemistry assays in cortex and hippocampus showed co-expression of all isoforms in
the neuropil associated with synapses and in the plasma membrane of pyramidal cells soma, while
cerebellum showed a more isoform-specific distribution pattern in Purkinje cells.
Conclusion: These results show an upregulation of PMCA activity and PMCA isoforms expression
during brain development in mouse, with specific localizations mainly in cerebellum. Overall, our
findings support a close relationship between the ontogeny of PMCA isoforms and specific
requirements of Ca2+ during development of different brain areas.
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