Design of clone-specific probes from genome sequences for rapid PCR-typing of outbreak pathogens.

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Abstract

The genome sequence of one OXA-48-producing Klebsiella pneumoniae belonging to sequence type (ST) 405, and three belonging to ST11, were used to design and test ST-specific PCR assays for typing OXA-48-producing K. pneumoniae. The approach proved to be useful for in-house development of rapid PCR typing assays for local outbreak surveillance.

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López Camacho, E., Rentero, Z., Ruiz-Carrascoso, G., et al. (2014). Design of clone-specific probes from genome sequences for rapid PCR-typing of outbreak pathogens. Clinical Microbiology and Infection, Vol. 20, Núm. 12, pp. 891-893.