Regulation of flotillin-1 in the establishment of NIH-3T3 cell–cell interactions.

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URI: https://hdl.handle.net/10641/5545
ISSN: 0014-5793
DOI: 10.1016/S0014-5793(03)01239-0

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2003

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Wiley
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Abstract

Flotillin-1 is a lipid raft-associated protein involved in neuronal regeneration, insulin signaling in adipocytes, and phagosome maturation in macrophages. We detected abundant flotillin-1 mRNA expression in confluent cultures of mouse NIH-3T3 fibroblasts. These cells were used as a model to assess the regulation of flotillin-1 mRNA and protein expression by cell–cell interactions. The disruption of cell–cell interactions triggered the downregulation of the flotillin-1 gene. However, the establishment of new cell interactions, and finally confluence, increased both the flotillin-1 transcript and its protein. Despite flotillin-1 having been described as an integral protein of the cell membrane, it is also very conspicuous in the hydrophilic fraction (the non-membrane fraction) of confluent cell extracts. The promoter region of the flotillin-1 gene was isolated and analyzed by transfection experiments, and the regulatory domains containing motifs for binding transcription factors involved in cell differentiation were identified.

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Flotillin-1, Reggie-2, Lipid raft, Cell–cell interaction, Flo-1 promoter

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López Casas, P.P., del Mazo, J. (2003). Regulation of flotillin-1 in the establishment of NIH-3T3 cell-cell interactions. FEBS Letters, 555(2), pp. 223-8.

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FACULTAD DE MEDICINA