Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization.

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1-s2.0-S2666166722006359-main.pdf (3.27 MB)
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URI: https://hdl.handle.net/10641/3362
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2022.101755

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2022

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In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and b-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches.

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Cell biology, Cell culture, Flow cytometry/Mass cytometry, Health sciences, Immunology, Model organisms

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FACULTAD DE CIENCIAS EXPERIMENTALES