The Calcium Signal for Balb/MK Keratinocyte Terminal Differentiation Induces Sustained Alterations in Phosphoinositide Metabolism without Detectable Protein Kinase C Activation.
dc.contributor.author | Moscat, Jorge | |
dc.contributor.author | Fleming, T P | |
dc.contributor.author | Molloy, C J | |
dc.contributor.author | López Barahona, Mónica | |
dc.contributor.author | Aaronson, S A | |
dc.date.accessioned | 2024-01-23T11:38:57Z | |
dc.date.available | 2024-01-23T11:38:57Z | |
dc.date.issued | 1989 | |
dc.description.abstract | Balb/MK keratinocytes require epidermal growth factor for proliferation and terminally differentiate in response to elevated extracellular Ca2+ concentrations. The molecular pathways controlling cell differentiation in this system have yet to be established. We show that a dramatic and sustained activation of phosphoinositide metabolism is produced upon addition of Ca2+ to Balb/MK cultures. The pattern of inositol trisphosphate isomers released in response to Ca2+ challenge appeared to be atypical. Inositol 1,3,4-trisphosphate release was observed by 30 s and was produced earlier than any alteration in inositol 1,4,5-trisphosphate levels. Concomitant with the liberation of inositol phosphates, an increased production of diacylglycerol was observed. Despite a 3-fold increase in diacylglycerol levels detected even at 12 h after Ca2+ addition, no evidence of functional activation or down-regulation of protein kinase C was found. This was established by measuring p80 phosphorylation, epidermal growth factor binding, and protein kinase C levels by immunoblotting. Analysis of the diacylglycerol generated following Ca2+ addition to Balb/MK cells revealed that a significant proportion of that lipid was an alkyl ether glyceride molecular species. Therefore, it is possible that this diacylglycerol molecular species may play a role in the Ca2+-induced differentiation program of Balb/MK cells through mechanisms other than stimulation of classical protein kinase C. | spa |
dc.description.extent | 4261 KB | spa |
dc.identifier.doi | 10.1016/S0021-9258(18)60453-3 | spa |
dc.identifier.issn | 0021-9258 | spa |
dc.identifier.uri | https://hdl.handle.net/10641/3796 | |
dc.language.iso | eng | spa |
dc.publisher | Journal of Biological Chemistry | spa |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0021925818604533 | spa |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.accessRights | open access | spa |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.title | The Calcium Signal for Balb/MK Keratinocyte Terminal Differentiation Induces Sustained Alterations in Phosphoinositide Metabolism without Detectable Protein Kinase C Activation. | spa |
dc.type | journal article | spa |
dc.type.hasVersion | AM | spa |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | f84ad2f1-2f78-4bff-b265-12fd8872e8e3 | |
relation.isAuthorOfPublication.latestForDiscovery | f84ad2f1-2f78-4bff-b265-12fd8872e8e3 |
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