Mixtures of Pseudomonas putida CECT 5279 cells of different ages: optimization as biodesulfurization catalyst.
Abstract: Sulfur contentinfossilfuelsisknowntobethemostimportantanthropogeniccauseofsulfuroxide
emissions totheatmosphere.Inordertoavoidhealth,environmentalandtechnicalproblemscaused
by thiscompound,legislationimposesrestrictivelimitationstofuelsulfurcontent.Biodesulfurization
(BDS) canbecomeacomplementarytechnologytohydrodesulfurization(HDS)tofacethissituation.
Pseudomonas putida CECT 5279isageneticallymodifiedmicroorganismwhichcanactasadesulfurizing
biocatalyst. Thismicroorganismhastheabilityofperformingthemetabolic4Spathwayfrom Rhodococcus
erythropolis IGTS8, inordertodesulfurizeDBT,asamodelcompound.Maximum in vivo activities of
monooxygenase enzymes(DszAandDszC)areshownwhenlateexponentialgrowthphaseisreached
(23 h),whiledesulfinaseenzymeDszBpresentsamaximumactivityduringtheearlyexponentialgrowth
phase (5h),aspreviouslyreported [1]. Also,ithasbeenprovedthatthecombinedutilizationofthesetwo
cell agesyieldsexcellentresultswhenusedasbiocatalystfordesulfurization [2]. Theaimofthisworkis
to optimizetheratioandtotalbiomassconcentrationofboth5hand23hgrowthtimecellsinacomplex
biocatalyst fordesulfurizationbyperformingrestingcellsbiodesulfurizationassaysusingdibenzothio-
phene (DBT)assulfurmodelcompound.Thebestcombinationofcellswasdeterminedaimingforthe
highest desulfurizationintheshortesttimeofoperationwhileinvestingtheminimumconcentrationof
biomass. Aparticularcellmixture,containing66.7%of23hgrowthtimecells,wasfoundtoworkasthe
most effectivedesulfurizationbiocatalyst.
Universal identifier: http://hdl.handle.net/10641/1002
Date: 2011
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